Effects of dexmedetomidine on postoperative pain and early cognitive impairment in older male patients undergoing laparoscopic cholecystectomy.

The primary aim of the present study was to investigate the effect of dexmedetomidine (DEX) on postoperative pain and early cognitive impairment in old male patients, who underwent laparoscopic cholecystectomy (LC). A total of 97 old patients, subjected to LC at the 980 Hospital of the Joint Service Support Force of the People's Liberation Army of China, were randomly divided into two groups, namely the DEX and normal saline groups. Patients in the DEX group received an intravenous infusion of 0.8 µg/kg DEX within 10 min following general anesthesia, followed by a maintenance infusion of 0.5 µg/(kg/h). Furthermore, patients in the normal saline group were treated with an equivalent volume of normal saline. Cognitive function was assessed using the Mini-Mental State Examination (MMSE) and the Montreal Cognitive Assessment (MoCA) tests at 6 h, 1, 2 and 3 days, postoperatively. The incidence of postoperative cognitive dysfunction (POCD) and postoperative adverse events were recorded for both groups. In addition, the Visual Analogue Scale (VAS) pain score was utilized to assess the pain level of all patients, while the Quality of Recovery-15 (QoR-15) scale was employed to analyze the postoperative recovery results. Therefore, the MoCA score was higher in the DEX group compared with the normal saline group at 6 h and day 1 postoperatively. Additionally, the MMSE score was higher at 6 h postoperatively in the DEX group compared with the normal saline group. Correspondingly, the incidence of POCD was lower in the DEX group compared with the normal saline group at 6 h and day 1, after LC (P<0.05). VAS score in resting state for patients in the DEX group was significantly lower compared with the normal-saline group (P<0.05). Furthermore, the QoR-15 scale score in patients in the DEX group was notably increased compared with the normal saline group on the first and second days after the operation (P<0.05). Overall, the present study verified that the continuous infusion of DEX at a rate of 0.5 µg/(kg/h) during LC could effectively reduce the incidence of early POCD and alleviate postoperative pain in old male patients, thus facilitating postoperative recovery.

A qualitative study exploring partner involvement in the management of gestational diabetes mellitus: The experiences of women and partners.

AIMS: The aims of the study were to explore the experiences of women with gestational diabetes mellitus (GDM) and their partners and examine the factors influencing partner involvement in GDM management, seeking to inform a targeted couple-based intervention. DESIGN: A descriptive qualitative study. METHODS: We conducted semi-structured interviews with 14 women with GDM and their partners. Participants were recruited through convenience sampling from a tertiary hospital in Xi'an, China. Data were analysed using thematic analysis. RESULTS: Three themes and 12 subthemes were identified. Theme I: Women's expectations of their partner's involvement in GDM management-practical support and emotional support. Theme II: Partner involvement in GDM management-constructive involvement, unhelpful involvement with good intentions and insufficient involvement. Theme III: Factors that influence partner involvement in GDM-knowledge of GDM, GDM risk perception, health consciousness, attitudes towards the treatment plan, couple communication regarding GDM management, family roles and appraisal of GDM management responsibility. CONCLUSION: Women desired practical and emotional support from partners. The types of partner involvement in GDM management varied. Some partners provided constructive support, while some partners' involvement was limited, non-existent or actively unhelpful. By combining these results with the factors influencing partner involvement, our findings may help healthcare professionals develop strategies to involve partners in GDM care and enhance women's ability to manage GDM. IMPLICATIONS FOR THE PROFESSION AND PATIENT CARE: Partner involvement in GDM care may help them understand and better attend to women's needs, thus improving their experience and potential outcomes. This study highlights novel factors that need to be considered in developing couple-based interventions for this population. REPORTING METHOD: The reporting follows the COREQ checklist. PATIENT OR PUBLIC CONTRIBUTION: Some patients were involved in data interpretation. There is no public contribution.

Effect of different inocula on the granulation process, reactor performance and biodiesel production of algal-bacterial granular sludge (ABGS) under low aeration conditions.

The algal-bacterial granular sludge (ABGS) system is a prospective wastewater treatment technology, but few studies focused on the effects of different inoculum types on the establishment of the ABGS system under low aeration conditions (step-decrease superficial gas velocity from 1.4 to 0.5 cm/s). Results from this study indicated that compared with other inocula, the ABGS formed by co-inoculating aerobic granular sludge (AGS) and targeted algae (Chlorella) exhibited a shorter granulation period (shortened by 15 days), higher total nitrogen (89.4%) and PO43--P (95.0%) removal efficiencies, and a greater yield of fatty acid methyl esters (FAMEs) (9.04 mg/g MLSS). This was possibly attributed to that the functional bacteria (e.g. Thauera, Gemmobacter and Rhodobacter) in the inoculated AGS facilitated the ABGS granulation. The inoculated algae promoted their effective enrichment under illumination conditions and enhanced the production of extracellular polymeric substances, thus improving the stability of ABGS. The enriched algae were attached to the outer layer of the granules, which could provide sufficient oxygen for bacterial metabolism, revealing the inherent mechanisms for the good stability of ABGS under low aeration intensity. Overall, the rapid granulation of ABGS can be achieved by inoculating optimal inocula under low aeration conditions, which is convenient and economically feasible, and motivates the application of algal-bacterial consortia.

The effects of a couple-based gestational diabetes mellitus intervention on self-management and pregnancy outcomes: A randomised controlled trial.

AIMS: To estimate the effectiveness of the Couples Coping with Gestational Diabetes Mellitus (GDM) Programme on GDM self-management and pregnancy outcomes. METHODS: A randomised controlled trial among pregnant women with suboptimal GDM self-management and their partners was undertaken. Couples recruited from three hospitals in China were randomly allocated to either intervention (n = 70) or control (n = 70) conditions. Couples in the intervention group underwent the couple-based intervention (GDM education, shared illness appraisals, initiation of collaborative action and consolidation of collaborative action). Women in the control group received individual GDM education. Data were analysed using the independent samples t-test, chi-square test, and generalised estimating equations. RESULTS: GDM knowledge for the women and their partners and GDM self-management significantly improved in both the intervention and control groups, with stronger improvement in the intervention group. Women in the intervention group gained significantly less weight than those in the control group (11.2 kg ± 2.8 kg vs 13.1 kg ± 2.6 kg, p = 0.008). Infant birth weights were significantly lower in the intervention group (3.2 kg ± 0.3 kg vs 3.4 kg ± 0.4 kg, p = 0.008). There were no significant differences in other pregnancy outcomes. CONCLUSIONS: The Couples Coping with GDM Programme was associated with improvements in GDM knowledge of women and their partners and in women's self-management, and with lower gestational weight gain and infant birth weight.

Human umbilical cord mesenchymal stem cell­derived exosomes improve ovarian function in natural aging by inhibiting apoptosis.

Prolonging the reproductive lifespan is beneficial for preserving the physical and psychological health of women. The transplantation of mesenchymal stem cell (MSC)­derived exosomes (MSC­Exos) has been reported to be a promising regenerative therapeutic strategy for restoring the function of aging ovaries. The present study thus evaluated the therapeutic efficacy of exosomes derived from human umbilical cord­MSCs (hUCMSC­Exos) in a mouse model of natural ovarian aging (NOA), and further investigated the role of exosomal microRNAs (miRNAs/miRs) in the mechanisms of this creative therapy. Specifically, following the administration of hUCMSC­Exos in mice with NOA, ovarian function was found to improve, as indicated by the restoration of follicle numbers and hormone levels. These exosomes were found to exhibit the ability to inhibit PTEN expression and suppress apoptosis both in vivo and in vitro. Subsequently, miRNA sequencing of the exosomes was performed, following which bioinformatics analysis was used to identify the highly expressed miRNAs that are capable of targeting PTEN expression. Through high­throughput sequencing and molecular analyses, miR­21­5p was found to be the highest in ranking in terms of expression, suggesting that hUCMSC­Exos can preserve ovarian function by suppressing PTEN expression to inhibit apoptosis by delivering miR­21­5p. On the whole, the results of the present study suggest that the application of exosomes can be used to restore ovarian function in mice with NOA. These positive findings also suggest that the transplantation of exosomes derived from MSCs holds promise as an agent against ovarian aging.

Adaptation and validation of the Evidence-based Practice Profile Questionnaire (EBP2Q) for clinical postgraduates in a Chinese context.

BACKGROUND: Evidence-based practice (EBP) is an essential approach of optimizing patient outcomes and driving progress in clinical practice. As an important reserve talent of medical staff and researchers, the clinical postgraduates are expected to become the backbones of supporting the implementation of EBP in clinical units after graduation. The assessment of their EBP learning outcomes is an important issue, yet few tools have been developed specifically in Mainland China. The purpose of this study is to adapt the Evidence-Based Practice Profile Questionnaire (EBP2Q) to Mainland China's cultural context and to evaluate the psychometric properties of the Chinese EBP2Q in clinical postgraduates. METHODS: Cross-cultural modification, including translating the original EBP2Q into Chinese was implemented according to established guidelines. A pilot study was carried out in Mainland China among 30 clinical postgraduates. A subsequent validation study was conducted among 633 clinical postgraduates majoring in clinical medicine, stomatology and nursing from Mainland China. Construct validity was assessed by exploratory factor analysis (n = 313), together with confirmatory factor analysis (n = 320). Reliability was determined by internal consistency and test-retest reliability. RESULTS: The Chinese EBP2Q consisted of 40 items. The content validity index of the Chinese EBP2Q achieved 0.938 at an acceptable level. Principal component analysis resulted in a four-factor structure explaining 61.586% of the total variance. All fitting indices satisfied the standard based upon confirmatory factor analyses, indicating that the four-factor structure contributed to an ideal model fit. The internal consistency appeared high for the Chinese EBP2Q, reaching a Cronbach's alpha value of 0.926. Test-retest reliability was 0.868 and the split-half coefficient was 0.925. CONCLUSION: Chinese version of EBP2Q possesses adequate validity, test-retest reliability and internal consistency. It is a promising questionnaire to be adopted by Chinese medical educators in designing their course and curriculum, or by clinical postgraduates for self-assessment of EBP learning.

A novel membrane bioreactor inoculated with algal-bacterial granular sludge for sewage reuse and membrane fouling mitigation: Performance and mechanism.

In this study, a novel membrane bioreactor (MBR) inoculated with algal-bacterial granular sludge (ABGMBR) was established to improve pollutant removal and alleviate membrane fouling. The ABGMBR system showed higher pollutant removal rate and longer operation time (152 day) compared to the control MBR (AGMBR). Moreover, the contents of the pollutants such as granular sludges, extracellular polymeric substances (EPS), and soluble microbial products on the membrane were remarkably reduced, leading to the formation of a porous and loose cake layer on the membrane and a slow increase in transmembrane pressure. Standard blocking was the main mechanism of membrane fouling; however, the membrane pore blockage was significantly reduced in ABGMBR. The extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory suggested that the aggregation and adhesion of foulants on the membrane were greatly inhibited in ABGMBR. Furthermore, correlation analysis showed significant differences in membrane fouling characteristics between AGMBR and ABGMBR. The ABGMBR system effectively retarded sludge disintegration and increased the repulsion between the sludge and membrane owing to the favorable mixed liquor characteristics. This study showcases the superior operational efficiency and anti-fouling performance of ABGMBR, offering a novel perspective on sewage reuse and membrane fouling mitigation.

Metagenomic analysis reveals the responses of microbial communities and nitrogen metabolic pathways to polystyrene micro(nano)plastics in activated sludge systems.

Microplastics (MPs) and nanoplastics (NPs) are prevalent in sewage and pose a potential threat to nitrogen biotransformation in wastewater treatment systems. However, investigations on how MPs and NPs affect the microbial nitrogen conversion and metabolism of the activated sludge are still scanty. Herein, the responses of microbiomes and functional genes to polystyrene MPs and NPs in activated sludge systems were investigated by metagenomic analysis. Results indicated that 1 mg/L MPs and NPs had marginal impacts on the nitrogen removal performance of the activated sludge systems, whereas high concentrations of MPs and NPs (20 and 100 mg/L) decreased the total nitrogen removal efficiency (13.4%-30.6%) by suppressing the nitrogen transformation processes. Excessive reactive oxygen species induced by MPs and NPs caused cytotoxicity, as evidenced by impaired cytomembranes and decreased bioactivity. Metagenomic analysis revealed that MPs and NPs diminished the abundance of denitrifiers (e.g. Mesorhizobium, Rhodobacter and Thauera), and concurrently reduced the abundance of functional genes (e.g. napA, napB and nirS) encoding for key enzymes involved in the nitrogen transformations, as well as the genes (e.g. mdh) related to the electron donor production, thereby declining the nitrogen removal efficiency. Network analysis further clarified the attenuate association between denitrifiers and denitrification-related genes in the plastic-exposed systems, elucidating that MPs and NPs restrained the nitrogen removal by inhibiting the contributions of microorganisms to nitrogen transformation processes. This study provides vital insights into the responses of the microbial community structure and nitrogen conversion processes to micro(nano)plastics disturbance in activated sludge systems.

Small intestinal submucosa promotes angiogenesis via the Hippo pathway to improve vaginal repair.

Vaginal reconstruction has incorporated the use of gastrointestinal segments for decades, but the technique is inevitably associated with complications. Tissue-engineering techniques, however, have brought great hope for vaginal reconstruction. This study aimed to evaluate the utility of small intestinal submucosa (SIS) in reconstructing clinically significant large vaginal defects in a porcine model and to investigate the role of the Hippo pathway in the vascular remodeling process. The composition and mechanical properties of SIS were characterized. Full-thickness vaginal defects were established in 10 minipig donors, with 4 cm lengths removed and replaced by an equal sized SIS scaffolds. The neovaginas were subjected to macroscopic, histological, immunohistochemical and molecular evaluations at 4 and 12 weeks after the surgery. Four weeks after the operation, extracellular matrix reorganization and complete coverage of the surface of the luminal matrix by vaginal epithelium were observed, accompanied by the formation of a microvascular network and the regeneration of smooth muscles, albeit disorderly arranged. Twelve weeks after implantation, enhancements were seen in the formation of the multi-layered squamous epithelium, angiogenesis, and large muscle bundle formation in the vagina. Additionally, the expression levels of angiogenesis-related proteins, proliferation-related proteins and Hippo pathway-related proteins in the neovagina were significantly increased. These results indicate that SIS could be used to reconstruct large vaginal defects and that the vascular remodeling process is potentially regulated by the Hippo pathway.

Variations in trihalomethane formation potential of Microcystis aeruginosa under different growth conditions: Phenomenon and mechanism.

Cyanobacteria and their metabolites are one of the primary precursors of disinfection by-products (DBPs) in natural water environments. However, few studies have investigated whether the production of DBPs by cyanobacteria changes under complex environmental conditions and possible mechanisms underlying these changes. Therefore, we investigated the effects of algal growth phase, water temperature, pH, illumination and nutrients on the production of trihalomethane formation potential (THMFPs) by Microcystis aeruginosa in four algal metabolic fractions, that is, hydrophilic extracellular organic matter (HPI-EOM), hydrophobic EOM (HPO-EOM), hydrophilic intracellular organic matter (HPI-IOM) and hydrophobic IOM (HPO-IOM). Additionally, correlations between THMFPs and some typical algal metabolite surrogates were analyzed. The results showed that the productivity of THMFPs by M. aeruginosa in EOM could be affected significantly by the algal growth phase and incubation conditions, while the IOM productivity varied insignificantly. M. aeruginosa in the death phase could secrete more EOM and have a higher THMFP productivity than those in the exponential or stationary phases. Cyanobacteria grown under harsh conditions could have increased THMFP productivity in EOM by increasing the reactivity of algal metabolites with chlorine, for example, under low pH conditions, and secreting more metabolites in EOM, for example, under low temperature or nutrient limitation conditions. Polysaccharides were responsible for the enhanced THMFP productivity in HPI-EOM fraction, and a significant linear correlation was found between the concentration of polysaccharides and THMFPs (r = 0.8307). However, THMFPs in HPO-EOM did not correlate with dissolved organic carbon (DOC), ultraviolet absorbance at 254 nm (UV254), specific UV absorbance (SUVA) and cell density. Thus, we could not specify the kind of algal metabolites that contribute to the increased THMFPs in the HPO-EOM fraction under harsh growth conditions. Compared with the case in EOM, the THMFPs in IOM were more stable and correlated with the cell density and total amount of IOM. The results implied that the THMFPs in the EOM were sensitive to growth conditions and were independent of algal density. Considering the fact that traditional water treatment plants cannot remove dissolved organics as efficiently as algal cells, the increased THMFP productivity in EOM by M. aeruginosa under harsh growth conditions could be a potentially serious threat to the safety of the water supply.

Identification of ADA as a Biomarker for Atypical Epstein Barr Virus Infection in Children.

OBJECTIVE: This study aims to explore the ability of adenosine deaminase (ADA) to discriminate atypical Epstein Barr virus (EBV) infection in children from acute febrile illness. METHODS: All children admitted to the Children's Hospital of Soochow University between 2018 and 2019, who were acute febrile patients and subjected to the plasma EBV-DNA polymerase chain reaction (PCR) and indirect immunofluorescence (IIF) assay for EBV-specific antibodies assays. The diagnostic value of each detection index was compared by the area under the ROC curve. RESULTS: In children with atypical Epstein Barr virus infection, the sensitivity, specificity, positive predictive value, negative predictive value and Youden index were 62.87%, 100.00%,100.00%, 61.73% and 0.63 for EBV-DNA PCR assay, 80.84%, 100.00%, 100.00%, 75.76% and 0.81 for VCA-IgG avidity and 89.22%, 87.00%, 91.98%, 82.86% and 0.76 for ADA. VCA-IgG avidity (AUC=0.904, P<0.01) and ADA (AUC=0.881, P<0.01) assays had the great diagnostic efficiency. In addition, the sensitivity, specificity and AUC were 92.75%,91.43% and 0.921(95%CI: 0.856-0.985) for ADA in the course≤3 days group, respectively. CONCLUSIONS: ADA has a good diagnostic value in the early stage of atypical EBV infection, and is not affected by primary EBV infection and reactivation. SCHLüSSELWöRTER: Adenosine deaminase, Epstein -Barr virus, Biomarker, children.

Rapid establishment of algal-bacterial granular sludge system by applying mycelial pellets in a lab-scale photo-reactor under low aeration conditions: Performance and mechanism analysis.

Light-driven algal-bacterial granular sludge (ABGS) is an innovative low-carbon technology with significant merits in treating municipal wastewater, but how to shorten the photogranulation process, especially under low aeration conditions, is largely unknown. Herein, two strategies were proposed to accelerate the start-up of the ABGS system in photo-sequencing batch reactors (PSBRs) with a low superficial gas velocity of 0.5 cm/s. Compared to directly dosing mycelial pellets (MPs), applying MPs to flocculate algae and using the formed algal-mycelial pellets (AMPs) as carriers enhanced the establishment of the algal-bacterial symbiosis. The ABGS system developed rapidly within 20 days, with a large particle diameter (mean diameter of 321 µm) and excellent settleability (SVI30 of 55.4 mL/g). More importantly, this system could be stably operated for at least 100 days, mainly attributed to the reinforced secretion of protein with unique secondary structure and elevated hydrophobic functional groups. As for the reactor performance, the average removal efficiencies of the ABGS system were 97.8% for organic matter, 80.0% for total nitrogen, and 84.4% for phosphorus. The enrichment of functional bacteria and algae, and the up-regulation of functional genes and enzymes involved in electron production and transport processes likely drove the transformation of the pollutants, underlining the inherent mechanism for the excellent nutrient removal performance. This study provides a promising approach to solve the problem of a long ABGS start-up period and unstable granular structure under low aeration conditions, which is significant for achieving effective wastewater treatment without energy intensive aeration.

Low salinity enhances azo dyes degradation in aerobic granular sludge systems: Performance and mechanism analysis.

The biodegradation performance of azo dyes can be enhanced under low salinity conditions, but the internal biodegradation mechanism is still unclear. Aerobic granular sludge (AGS), a salt-tolerant biological wastewater treatment technology, was used in this study to explore the enhancement mechanism of acid orange 7 (AO7) degradation at low salinity level (1.0 %). Results indicated that the AGS structure and reactor performance were almost unaffected by different AO7 concentrations (5-10 mg/L). Compared with salt-free conditions, the AO7 removal efficiency was elevated by 9.9 %-19.0 % at 1.0 % salinity level, owing to the enrichment of AO7 decolorizing bacteria (e.g. Acinetobacter) and functional enzymes (e.g. FMN-dependent azoreductase). The up-regulated genes involving in the key metabolic functions (e.g. carbon metabolism and oxidative phosphorylation) promoted the electron and energy production, thereby facilitating the AO7 decolorization and degradation. These results aid understanding of the enhancement mechanism of AO7 biodegradation under low salinity conditions from macroscopic and microscopic perspectives.

The female germ unit is essential for pollen tube funicular guidance in Arabidopsis thaliana.

In angiosperm, two immotile sperm cells are delivered to the female gametes for fertilization by a pollen tube, which perceives guidance cues from ovules at least at two critical sites, micropyle for short-distance guidance and funiculus for comparably longer distance guidance. Compared with the great progress in understanding pollen tube micropylar guidance, little is known about the signaling for funicular guidance. Here, we show that funiculus plays an important role in pollen tube guidance and report that female gametophyte (FG) plays a critical role in funicular guidance by analysis of a 3-dehydroquinate synthase (DHQS) mutant. Loss function of DHQS in FG interrupts pollen tube funicular guidance, suggesting that the guiding signal is generated from FG. We show the evidence that the capacity of funicular guidance is established during FG functional specification after the establishment of cell identity. Specific expression of DHQS in the synergid cells, central cells, or egg cells can rescue funicular guidance defect in dhqs/+, indicating all the female germ unit cells are involved in the funicular guidance. The finding reveals that the attracting signal of pollen tube funicular guidance was generated at a site and stage manner and provides novel clue to locate and search for the signal.

Biotransformation of sulfamethoxazole (SMX) by aerobic granular sludge: Removal performance, degradation mechanism and microbial response.

Aerobic granular sludge (AGS) is a promising biotechnology for the treatment of antibiotic-rich wastewater. However, little is known about the antibiotics degradation mechanism and microbial response in a sulfamethoxazole (SMX)-loaded AGS system. Herein, the results of a continuous 240 days test suggested that 0.5-5 mg/L of SMX could be thoroughly removed by AGS via adsorption and degradation. The degradation pathway of SMX involved the hydrolysis of the sulfonamide bond and cleavage of NS or CS bonds, subsequently leading to the production of small molecular substances (e.g. benzene and 5-methyl-isoxazole). In terms of the AGS system, it exhibited a strong resistance to 0.5 mg/L of SMX, while 1 and 5 mg/L of SMX significantly inhibited the microbial growth, declined the nitrification efficiency, weakened the sludge settleability, and triggered the excessive growth of filamentous bacteria. Besides, the secretion of extracellular polymer substances was suppressed by 57.3% when increasing the SMX concentration from 0.5 to 5 mg/L, which was not conducive to the system stability. The long-term presence of SMX enhanced the proliferation of antibiotics resistance genes (sul1and sul2) and exerted a strong selection pressure on the microbial community, especially with Thiothrix being the dominating genus. Overall, this study elucidated that AGS qualified promising application prospects in the removal of SMX present in wastewater, but SMX at high concentrations posed great adverse impacts on the performance of the AGS system, which causes concern when treating SMX rich wastewaters.

Drug-free in vitro activation combined with 3D-bioprinted adipose-derived stem cells restores ovarian function of rats with premature ovarian insufficiency.

BACKGROUND: Emerging drug-free in vitro activation (IVA) technique enables patients with premature ovarian insufficiency (POI) to restore ovarian function and conceive their own genetic offspring. However, various issues have greatly restricted its clinical application. Transplantation of adipose-derived stem cells (ADSCs) has promising roles in restoring ovarian function of rats with POI, but insufficient retention has greatly hampered their efficiency. Here, we designed a 3D-bioprinted engineering ovary composed of drug-free IVA and ADSCs, which may prolong the retention of ADSCs and construct an early vascular microenvironment, thus compensating for the disadvantages of drug-free IVA to some extent and ameliorating impaired ovarian function in the POI rats. METHODS: After intraperitoneal injection of cyclophosphamide, the POI model rats were randomized into 5 groups: (1) POI group; (2) ovarian fragments group; (3) 3D scaffold combined with ovarian fragments group; (4) ovarian fragments combined with ADSCs group; (5) 3D scaffold with ADSCs combined with ovarian fragments as 3D-bioprinted engineering ovary group. Normal rats were identified as the control group. The localization of CM-Dil-labeled ADSCs and co-localization with CD31 were observed to examine the distribution and underlying mechanism of differentiation. Histomorphological and immunohistochemical analyses were performed to calculate follicle number and assess proliferation and apoptosis of granulosa cells (GCs). Immunofluorescence staining was used to evaluate angiogenesis. Hormone levels were measured to evaluate the restoration of endocrine axis. Western blot analysis and RT-PCR were conducted to explore the potential mechanism. RESULTS: CM-Dil-labeled ADSCs were distributed in the interstitium of ovaries and had significantly higher retention in the 3D-bioprinted engineering ovary group. Several regions of the co-staining for CM-Dil and CD31 were in the area of vascular endothelial cells. Meanwhile, the follicle counts, GCs proliferation, neoangiogenesis, and hormone levels were significantly improved in the 3D-bioprinted engineering ovary group, as compared with other groups. Furthermore, the ovarian function was ameliorated and angiogenesis was promoted through regulating the PI3K/AKT pathway. CONCLUSION: Our results suggested that 3D-bioprinted engineering ovary had great potential for restoring impaired ovarian function of rats with POI, which could compensate for the disadvantages of drug-free IVA to some extent.

Degradation mechanisms of cyanobacteria neurotoxin ß-N-methylamino-l-alanine (BMAA) during UV254/H2O2 process: Kinetics and pathways.

In this work, the UV254/H2O2 process was utilized to remove ß-N-methylamino-l-alanine (BMAA), a kind of cyanobacteria neurotoxin, and the influence of reaction parameters and environmental factors on the degradation of BMAA has been systematically investigated. The results showed that BMAA could be effectively removed in the UV254/H2O2 system compared to UV or H2O2 alone and OH was confirmed as the main ROS to degrade BMAA. The degradation rate of BMAA increased first and then decreased with the increase of pH and the maximum kobs was 0.1545 min-1 obtained at pH 9. The removal of BMAA in the UV254/H2O2 system was inhibited in actual water, while the degradation rate of BMAA in actual water could still exceed 90% by appropriately extending the reaction time. The decrease in the degradation efficiency of BMAA in actual water was primarily due to the ultraviolet light absorption and competition effects of NOM, and anions (Cl- and HCO3-) would also inhibit the degradation of BMAA. Five by-products ([M - H]- = 118, 103, 88, 87 and 59) were identified in this study and the degradation pathways of BMAA were proposed. The production of by-products was attributed to the fracture of the C-N bond and hydroxylation reaction. This study is worthwhile to deepen the understanding of the degradation mechanism of BMAA in the UV254/H2O2 system.

Unravelling the biodegradation performance and mechanisms of acid orange 7 by aerobic granular sludge at different salinity levels.

Azo dyes wastewater is characterized by high-salinity, however, the biodegradation performance and mechanisms of azo dyes by aerobic granular sludge (AGS) under different salinity levels are still unclear. Herein, the results showed that the reactor performance was almost unaffected at low-salinity levels (0.5%-1.0% salinity), and the removal efficiency of acid orange 7 (AO7) was increased by 2.6%-19.1%, possibly due to the excessive secretion of extracellular polymeric substances (EPS) and the enrichment of functional bacteria. Nevertheless, the microbial cell viability was negatively affected by high-salinity level (2.0% salinity), leading to the deterioration of AO7 and nutrient removal efficiencies. The AO7 removal was achieved by rapid adsorption and slow biodegradation. The biodegradation pathway indicated that AO7 was gradually mineralized in the AGS system through desulfurization, deamination, decarboxylation and hydroxylation. Altogether, this work provides an important reference for the application of AGS technology for treating saline azo dye wastewaters.

Revealing the influencing mechanisms of polystyrene microplastics (MPs) on the performance and stability of the algal-bacterial granular sludge.

Algal-bacterial granular sludge (ABGS) is an energy-saving and environment-friendly wastewater treatment technology; however, the effects of microplastics (MPs) on the performance and stability of the ABGS system remain unknown. Herein, the influencing mechanisms of polystyrene MPs (50 µm) on the ABGS were systematically investigated. The ABGS exhibited a high removal efficiency of MPs (over 96%) at 1 mg/L and 20 mg/L. Although the biomass content, sludge settling and particle size were not obviously affected by MPs, the COD and total phosphorus (TP) removal efficiencies were inhibited by 2.6%-4.1% and 2.9%-5.8%, respectively. Meanwhile, the structural stability of ABGS was damaged by MPs, owing to the excessive oxidative stress, low content of protein-like substance (especially tryptophan and tyrosine), and the large portion of loose protein secondary structure. Microbial community analysis revealed that the relative abundance of some functional bacteria (Candidatus_Competibacter and Rhodobacter) and algal species (Tetradesmus) were decreased under the MPs stress.